Species:

Mouse

Alternative Name:

Carboxypeptidase E,CPE,Carboxypeptidase H (CPH),Enkephalin convertase,Prohormone-processing carboxypeptidase,Cpe

Uniport ID:

Q00493

Assay Type:

Sandwich

unit:

pg/mL

Standard:

1000pg/mL

Sensitivity:

9.38 pg/mL

Detection Range:

15.63-1000pg/mL

Assay Time:

3.5H

Sample Type:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL

Research:

Signal transduction;Developmental science;Bone metabolism;

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CPE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CPE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CPE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CPE.You can calculate the concentration of Mouse CPE in the samples by comparing the OD of the samples to the standard curve.